Please activate JavaScript!
Please install Adobe Flash Player, click here for download
ePaper created 2014-09-15, 12:31:02 | version 1.32.01
elasticity meters to quantify fibrosis in wound healing and in systemic sclerosis.[4,5] In patients with severe scleroderma treated with extracorporal photopheresis, this expertise was used to quantify fibrosis.[5] One of the main research goals was the development of an epidermal and dermal skin substitute, a cultured multilayer skin equivalent to be used in chronic ulcers, and especially in burn wounds. Many dermal substitutes covered with autologous keratinocytes were evaluated in tissue cultures, animal studies and in humans.[6,7,8] The skin equivalent research line was developed in close collaboration with Dutch burns units. Related subjects were scar formation, keloïd, hypertrophic scars and wound contraction in burns. Delayed wound healing in diabetic ulcers was investigated and the distribution of extracellular matrix proteins in diabetic ulcers in various stages of healing was assessed.[9,10] Fibroblasts from non-healing diabetic ulcers and controls were cultured and their proliferative response to growth factors were measured. The fibroblasts of diabetic patients showed a reduced response to growth factors.[10] References 1. Mol MAE, Westerhof W, Nanninga PB, et al. Grafting of venous leg ulcers: an intra-individual comparison between cultured skin equivalents and full thickness punches. J Amer Acad Dermatol 1991; 24: 77-82. 2. Poole IC le, Das PK, Krieg SR, et al. Organotypic culture of human skin for studying wound healing. Wounds: A Compendium Clin Res Pract 1991; 3: 102-10. 3. Mekkes JR, Zeegelaar JE, Westerhof W. Quantitative and objective evaluation of wound debriding properties of collagenase and fibrinolysin/desoxyribonuclease in a necrotic ulcer animal model. Arch Dermatol Research 1998; 290: 152-7. 4. Enomoto DNH, Mekkes JR, Bossuyt PMM, et al. Quantification of cutaneous sclerosis in patients with generalized scleroderma with a skin elasticity meter. J Amer Acad Dermatol 1996; 35: 381-7. 5. Enomoto DNH, Schellekens PTA, Yong S, et al. Extracorporeal Photochemotherapy (photophoresis) induces apoptosis in lymphocytes: a possible mechanism of action of PUVA therapy. Photochem Photobiol 1997; 65: 177-180. 6. Middelkoop E, De Vries HJC, Ruuls-van Stalle L, et al. Adherence, proliferation and collagen turnover by human fibroblasts seeded into different types of collagen sponges. Cell Tissue Res 1995; 280: 447-53. 7. Vries HJC de, Middelkoop E, Van Heemstra-Hoen M, et al. Stromal cells from subcutaneous adipose tissue seeded in an native collagen/elastin dermal substitute reduce wound contraction in full thickness skin defects. Lab Invest 1995: 73: 532-540. 8. Lamme EN, van Leeuwen RTJ, Jonker A, et al. Living skin substitutes : survival and function of fibroblasts seeded in a dermal substitute in experimental wounds. J Invest Dermatol 1998; 111: 989-95. 9. Loots MAM, Lamme EN, Zeegelaar JE, et al. Differences in cellular infiltrate and extracellular matrix of chronic diabetic and venous ulcers versus acute wounds. J Invest Dermatol 1998; 111: 850-7. 10. Loots MAM, Lamme EN, Mekkes JR, et al. Cultured fibroblasts from chronic diabetic wounds on the lower extremity (non-insulin-dependent diabetes mellitus) show disturbed proliferation. Arch Dermatol Res 1999; 291: 93-9. 45 BWEADVSMGFINCORR:Opmaak 1 21-07-2014 17:39 Pagina 45