• antibodies: structure and biological function, antibodies as tools in cellular biology • eukaryotic genomes: structure and function, chromosomes, mono- and diploid organisms • dna: structure and function, replication, repair, recombination • gene expression: mechanisms, expression enhancers, transcription factors, control, silencers • protein biosynthesis: from dna to proteins, ribosomes, regulation methodical focus • microscopy: light microscopy, fluorescence microscopy, confocal fluorescence microscopy • molecular biology: structure and function of plasmids, molecular cloning, polymerase chain reaction enzymes, transformation of bacteria restriction (pcr), • transgenic animals: creation of transgenic animals, knockdowns, knock-ins, knockouts, conditional knockouts, genome editing • protein biochemistry: immunological detection techniques, sds-page, western blot • cell culture: sterile working with mammalian transfection and expression of cell recombinant proteins, knockdown lines, goethe university | faculty of biosciences | institute of cell biology and neuroscience 6 | page